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You are at: Wagner Home > Technologies > Biotech > CATS Basecaller

CATS Basecaller

Description

CATS is a powerful basecalling software tool developed by Daniel H. Wagner Associates. CATS can read several chromatogram formats, work with raw or processed trace data, and determine basecalls and basecall locations. The tool is currently available on the Macintosh PowerPC platform.

The CATS algorithms were developed and tuned for optimal performance using capillary array electrophoretic traces from thousands of chromatograms from several sequencing labs. While specifically designed for CAE technology, CATS can be readily adapted to any sequencing technology that is based on electrophoretically derived fluorescent traces. Wagner Associates has the expertise on staff to adjust the software to meet your specific sequencing needs.

Features

  • Automatic computation of the color separation matrix
  • Automatic determination of optimal dye-mobility shift factors
  • Fourier analysis and bandpass filtering to improve signal quality
  • Dynamic programming approach balances the need for uniform peak spacing and intensity
  • Elementary confidence estimations to determine 5' and 3' cutoffs
    (see the ATQA home page for more sophisticated trace quality assessment tools)
  • Fast run time keeps pace with today's sequencing demands
  • Primer peak detection
  • Many user controllable parameters, including the following:
    • Can use a precomputed color separation matrix for increased efficiency
    • User adjustable dye-mobility shift factors
    • Bandpass filter allows user to select optimal frequency range
  • Accepts input in ABI and SCF formats
  • Produces output in SCF and ASCII formats

Contact Us

Please contact atqa@pa.wagner.com for further information on the CATS basecaller.  Go here for other contact options.


 

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