|
You are at: Wagner
Home > Technologies > Biotech
> ATQA > Sample ATQA Scores
Automated Trace Quality Assessment
(ATQA) for DNA Sequencing
Examples
of Quality Scores
| Substitution quality scores |
 |
 |
Basecall with high
substitution quality score |
Basecalls with low substitution
quality scores. (True sequence = GCTGTGTGCA) |
| Good substitution scores are characterized by
strong, consistent peaks, good color separation, uniform peak
spacing, and good peak shape at the base call location, as characterized
by the second derivative, for example. |
| Insertion quality scores |
 |
 |
Basecall with high
insertion quality score |
Basecall with low
insertion quality score |
| High insertion scores are characterized by strong,
consistent peaks, and good peak shape at the base call location,
and an average to more than average distance between peaks, when
compared to other calls in the same region. |
| Deletion quality scores |
 |
 |
Basecall with high
deletion quality score |
Basecall with low deletion
quality score. (True sequence = TAAAGAATACCA) |
| High deletion scores are characterized by the
absence of any strong peaks in the gap between two consecutive
basecalls, and an average to less than average distance between
peaks, when compared to other calls in the same region. |
Return to
Automated Trace Quality Assessment tool main page.
|
